Changes in Antimicrobial Susceptibility Pattern of Blood Isolates at a University Hospital in the Kyungnam area during 2005-2014

Article information

Kosin Med J. 2018;33(1):29-40

Publication date (electronic) : 2018 January 21

doi : https://doi.org/10.7180/kmj.2018.33.1.29

Department of Laboratory Medicine, College of Medicine, Kosin University Busan, Korea

Corresponding Author: Woonhyoung Lee, Department of Laboratory Medicine, College of Medicine, Kosin University, 262, Gamchen-ro, Seo-gu, Busan 49267, Korea Tel: +82-51-990-6373 Fax: +82-51-990-3034 E-mail: lukerubicon@gmail.com

Received 2017 January 12; 2017 February 27; Accepted 2017 March 15.

Abstract

Objectives

Blood culture is a one of the most important procedure for diagnosis and treatment of infectious disease, but distribution of pathogenic species and the antimicrobial susceptibility can be vary from pathogen, individual trait, regional or environmental features. In this study, we investigated the changes in frequency of occurrence and antimicrobial susceptibility pattern of blood isolates from 2005 to 2014.

Methods

Data of blood isolates from Kosin Gospel Hospital during 2005 to 2014 were analyzed retrospectively. Blood isolates were cultured for 5 days using BACTEC Plus Aerobic/F and BACTEC lytic/10 Anaerobic/F. Identification and antimicrobial susceptibility test was performed using VITEK 1 system, VITEK 2 XL, PHOENIX 100 and conventional method.

Results

9,847 isolates were identified during 10 years. Among the isolates aerobic or falcutative anaerobic bacteria were isolated in 99.5% specimens, anaerobic were 0.1%, and fugi were 0.4%. Most commonly isolated bacteria were coagulase-negative Staphylococcus (CoNS) followed by Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae. Candida parapsilosis were most frequently isolated among fungi. The proportion of S. aureus, A. baumannii and E. faecium were increased, while Pseudomonas aeruginosa and Streptococcus pneumoniae decreased over decennium. Imipenem resistant K. pneumoniae were identified. Vancomycin resistant E. faecium and imipenem resistant A. baumannii were increased (7.1% in 2005 to 12.3% in 2014, 0% in 2005 to 55.6% in 2014, respectively).

Conclusions

Over the last 10 year, CoNS were the most frequently isolated pathogen. Imipenem resistant K. pneumoniae was emerged. Vancomycin resistant E. faecium and imipenem resistant A. baumannii increased during this period.

Keywords: Antimicrobial susceptibility pattern; Bacteremia; Blood culture

Fig. 1.

Annual isolation of relatively common species of bacteria during 2005-2014

Fig. 2.

Trends in antimicrobial resistance (%) of (A) S. aureus, (B) E. faecalis, (C) E. faecium, (D) E. coli (E) K. pneumonia (F) P. aeruginosa and (G) A. baumannii by year.

Table 1.

Microorganisms isolated by year

Table 2.

Distribution of blood isolates

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