Kosin Medical Journal

Effects of cholecalciferol and omega-3 fatty acids on hepcidin levels in 5/6 nephrectomy rats: Yu In Jeong, Hyo Jin Jung, Mi Hwa Lee, Young Ki Son, Seong Eun Kim, Won Suk An, Su Mi Lee; Kosin Med J. 2024;39(1):35-43. Published online September 25, 2023; DOI: https://doi.org/10.7180/kmj.23.137

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Abstract PDF PubReader ePub: Background
Anemia is a common complication of chronic kidney disease (CKD). In patients with CKD-related anemia, an inverse relationship between vitamin D and hepcidin levels has been observed. Hepcidin is a key regulator of iron homeostasis, mediated via binding to ferroportin. The aim of this study was to investigate the effects of cholecalciferol and omega-3 fatty acids (FA) on hepcidin levels using 5/6 nephrectomized (Nx) rats.
Methods
Male Sprague-Dawley rats were divided into five groups: sham control, 5/6 Nx, 5/6 Nx treated with cholecalciferol, 5/6 Nx treated with omega-3 FA, and 5/6 Nx treated with both cholecalciferol and omega-3 FA. We measured the hepcidin and ferroportin levels in the kidney and liver by enzyme-linked immunosorbent assays and Western blots. We evaluated hepcidin expression in the kidney by immunohistochemical staining.
Results
Among the five groups, 5/6 Nx rats exhibited the worst kidney function. Compared with the sham controls, 5/6 Nx rats showed significantly increased serum hepcidin levels and decreased vitamin D levels. Supplementation with either omega-3 FA or cholecalciferol decreased hepcidin and increased vitamin D levels, with a concurrent improvement of anemia. Furthermore, 5/6 Nx rats treated with omega-3 FA/cholecalciferol showed decreased ferroportin and ferritin levels, while iron and total iron-binding capacity levels increased.
Conclusions
Treatment with a combination of cholecalciferol and omega-3 FA may improve anemia in a CKD rat model by decreasing hepcidin levels.

A Case of Aplastic Anemia Following Acute Hepatitis: Dae Sung Lee, Jung Hyun Lee, Jae Sun Park, Mi Hyang Kim; Kosin Med J. 2008;23(3):95-98. Published online September 30, 2008

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Bone Marrow Transplantation: Jae Who Park; The Journal of Kosin Medical College. 1990;6(1):87-96.

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A Case of Fanconi Anemia in an Infant with Early Hematologic Manifestation: Seong Hee Park, Kyeong Aa Lee, Jae Sun Park; The Journal of Kosin Medical College. 1992;8(2):225-230.

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A Case Report of Intestinal GIST in Von Recklinghausen's Disease: Seong Man Kim, Seong Hoon Chang, Yeon Soon Jung, Ho Sung Son, Han Ho Chung, Moo In Park, Seon Ja Park, Sung Uhn Baek, Ja Young Koo; Kosin Med J. 2000;15(1):90-93.

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Change of NK cell activity in case of iron deficiency anemia adult patients: Su Hong Kim, Jeoung Hoon Seo, Yang Soo Kim, Wan Gyu Eo; Kosin Med J. 2005;20(1):49-56.

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