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2 "Mee Sook Roh"
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Case report
Chronic obstructive lung disease after ammonia inhalation burns: a report of two cases
Insu Kim, Heock Lee, Bo Hyoung Kang, Dong Hyun Lee, Young Hee Nam, Mee Sook Roh, Soo-Jung Um
Kosin Med J. 2022;37(4):354-360.   Published online July 27, 2022
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Abstract PDFPubReader   ePub   CrossRef-TDMCrossref - TDM
Anhydrous ammonia is a commonly used chemical in industry. Ammonia gas inhalation causes thermal injuries and alkali burns in the airway and lung parenchyma. Previous case reports have stated that respiratory sequelae after acute ammonia inhalation burns were associated with structural lung disease, such as bronchiectasis or interstitial lung disease. We herein report two cases of long-term sequelae with persistent airflow limitation after ammonia inhalation burns.
Original article
Influence of Cold Ischemia Time and Storage Period on DNA Quality and Biomarker Research in Biobanked Colorectal Cancer Tissues
Min Gyoung Pak, Mee Sook Roh
Kosin Med J. 2020;35(1):26-37.   Published online June 30, 2020
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Abstract PDFPubReader   ePub   CrossRef-TDMCrossref - TDM

Biobanking plays an important role in future research. Assessment and control of the preanalytical variables of biobanked tissues are fundamentals for the optimal use of biospecimens.


Forty-five colorectal cancer (CRC) tissues stored at −80°C in Bio-Resource Bank were evaluated to define the influence of cold ischemia time (CIT) and storage period (SP) on DNA quality in biobanked tissues. Three CITs (less than 30 minutes (CIT-1), 30–45 minutes (CIT-2), and 45–60 minutes (CIT-3)) and three SPs (less than 1 year (SP-1), 2–3 years (SP-2), and 4–5 years (SP-3)) were chosen. NanoDrop spectrophotometer was used to determine the 260/280 ratio for DNA purity. DNA integrity was analyzed by a UV transilluminator following electrophoresis on 2% agarose gel. To evaluate the practical usability of DNA for biomarker research, KRAS mutation status was assessed by PCR amplification.


All DNA specimens had a 260/280 ratio ranging between 1.8 and 2.0 with the exception of one specimen (CIT-2/SP-2 group). For DNA integrity, DNA appeared as a compact, high-molecular-weight band with no or scanty low-molecular-weight smears. The concordance of KRAS mutation status between paired biobanked frozen tissues and formalin-fixed paraffin-embedded tissues was 100%. DNA remained stable in CRC tissues kept at room temperature for up to 1 hour and long-term storage up to 5 years.


Storage conditions of our biobank are suitable for long-term (at least five years) specimen preservation with high DNA quality. These results have practical implications that could affect banking guidelines.


Citations to this article as recorded by  
  • Advancing wide implementation of precision oncology: A liquid nitrogen‐free snap freezer preserves molecular profiles of biological samples
    Hanneke van der Wijngaart, Sahil Jagga, Henk Dekker, Richard de Goeij, Sander R. Piersma, Thang V. Pham, Jaco C. Knol, Babs M. Zonderhuis, Harry J. Holland, Connie R. Jiménez, Henk M. W. Verheul, Srinivas Vanapalli, Mariette Labots
    Cancer Medicine.2023;[Epub]     CrossRef

KMJ : Kosin Medical Journal